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Eco-Culture Medium
해조류 및 식물 성장을 돕기 위한
고농축 배양액
Seaweed & Phytoplankton
1. N; / 2. P / 3. K / 4. Ca; / 5. Mg; / 6. S; / 7. B; / 8. Cl; / 9. Cu;
10. Fe; / 11. Mn; / 12. Zn; / 13. Mo; / 14. Si / 15. Na / 16. Ni; / 17. Co;
※ 자세한 정보는 상담문의를 부탁드립니다.
1. NaNO3 / 2. K2HPO4 / 3. MgSO4·1H2O
4. CaCI2·2H2O / 5. Citric acid / 6. Ammonium ferric citrate green
7. Na2EDTA·2H2O / 8. Na2CO3 / 9. Vitamin
※ 자세한 정보는 상담문의를 부탁드립니다.
CONWAY Medium
NaNO3 100.0 g/L dH2O
Na2EDTA 45.0 g/L dH2O
N3BO3 33.6 g/L dH2O
NaH2PO4 · 2H2O 20.0 g/L dH2O
FeCl3 · 6H2O 1.30 g/L dH2O
MnCl2 · 4H2O 0.36 g/L dH2O
Trace Metal Solution 1 ml
Vitamin Mix 100 ml
Distilled water (to make) 1,000 ml
* Make final volume up to 1.0 L with filtered tertiary distilled water.
CONWAY Trace Metal Solution
ZnCl2 2.1 g/L dH2O
CoCl2 · 6H2O 2.1 g/L dH2O
(NH4)6 Mo7O24 · 4H2O 2.1 g/L dH2O
CuSO4 · 5H2O 2.0 g/L dH2O
* Distilled water 100㎖ (acidify with 1N HCl until solution is clear)
CONWAY Vitamin Solution
Vitamin B1 20mg
Vitamin B12 10mg
Distilled water 200㎖
References
Guillard R.R.L. and Ryther J.H. (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula
confervacea Cleve. Can. J. Microbiol. 8: 229-239.
Guillard R.R.L. (1975) Culture of phytoplankton for feeding marine invertebrates.
in “Culture of Marine Invertebrate Animals.” (eds: Smith W.L. and Chanley M.H.) Plenum Press,
New York, USA. pp 26-60.
Eco-Life & Technology
Dong-Han Lee(CEO). Researcher Lab Eco life of Aquatic Envronment physiology Rsearcher of Marine Bioscience and
Technology No. 306, CATHOLIC KWANDONG UNIVERSITY BUSINESS INCUBATION CENTER R&B Park,
Saimdang-ro, Gangeung-si, Ganwon-do, Repubic of korea, South KOREA
TEL: +82-10-9112-9351 FAX: +82-33-661-4888
MAIL: ehdgo914@naver.com www.lab-ecotec.co.kr
F/2 Medium
1.0 mL NaNO3 75.0 g/L dH2O
1.0 mL NaH2PO4.H2O 5.0 g/L dH2O
1.0 mL Na2SiO3.9H2O 30.0 g/L dH2O
1.0 mL f/2 Trace Metal Solution (see recipe below)
0.5 mL f/2 Vitamin Solution (see recipe below)
Make final volume up to 1.0 L with filtered tertiary distilled water.
Note: f/2 Medium contains extensive silica precipitate and should be used only
when growing diatoms. For other algal groups use f/2-Si Medium (see below).
F/2 Vitamin Solution
(Guillard & Ryther 1962, Guillard 1975)
To 950 mL dH2O add:
Quantity Compound Stock Solution
1.0 mL Vitamin B12 (Cyanocobalamin) 1.0 g/L dH2O
10.0 mL Biotin 0.1 g/L dH2O
200.0 mg Thiamine HCl - Make final volume up to 1.0 L with dH2O.
Filter sterilize into plastic vials and store in refrigerator.
References
Guillard R.R.L. and Ryther J.H. (1962) Studies of marine planktonic diatoms.
I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol. 8: 229-239.
Guillard R.R.L. (1975) Culture of phytoplankton for feeding marine invertebrates.
in “Culture of Marine Invertebrate Animals.” (eds: Smith W.L. and Chanley M.H.) Plenum Press, New York, USA. pp 26-60.
PES = Provasoli´s enriched Seawater Medium
General purpose marine medium for xenic cultures.
Preparation: add 1 tube (20 mL) of ES-enrichment to 1000 mL of pasteurized, filtered tertiary distilled water.
For ES-enrichment solution,
add the following to 100 ml glass-distilled water:
in 100 ml glass-distilled water
NaNO3 350 mg
Na2glycerophosphate .5 H2O 50 mg
Fe-solution 25 mL
PII metal-solution 25 mL
vitamin B12 10 µg
thiamine 0.5 mg
biotin 5 µg
Tris buffer (Sigma Co.) 500 mg
* Adjust to pH 7.8, dispense in tubes (20 mL/tube) and autoclave. Store at 10° C.
Fe-solution:
Dissolve 351 mg of Fe(NH4)2(SO4)2 . 6H2O and 300 mg of Na2EDTA in 500 mL of de-ionized or distilled water
PII metal solution:
in 100 ml glass-distilled water
Na2EDTA 100 mg
H3BO3 114 mg
FeCl3 6H2O 4.9 mg
MnSO4 H2O 16.4 mg
ZnSO4.7 H2O 2.2 mg
CoSO4. 7 H2O 0.48 mg
References
Provasoli L (1968). Media and prospects for the cultivation of marine algae. In A. Watanabe, A Hattori (eds),
Cultures and Collections of Algae. Japanese Society Plant Physiology, Hakone: 63-75.
1. N; / 2. P / 3. K / 4. Ca; / 5. Mg; / 6. S; / 7. B; / 8. Cl; / 9. Cu;
10. Fe; / 11. Mn; / 12. Zn; / 13. Mo; / 14. Si / 15. Na / 16. Ni; / 17. Co;
※ 자세한 정보는 상담문의를 부탁드립니다.
1. NaNO3 / 2. K2HPO4 / 3. MgSO4·1H2O
4. CaCI2·2H2O / 5. Citric acid / 6. Ammonium ferric citrate green
7. Na2EDTA·2H2O / 8. Na2CO3 / 9. Vitamin
※ 자세한 정보는 상담문의를 부탁드립니다.
CONWAY Medium
NaNO3 100.0 g/L dH2O
Na2EDTA 45.0 g/L dH2O
N3BO3 33.6 g/L dH2O
NaH2PO4 · 2H2O 20.0 g/L dH2O
FeCl3 · 6H2O 1.30 g/L dH2O
MnCl2 · 4H2O 0.36 g/L dH2O
Trace Metal Solution 1 ml
Vitamin Mix 100 ml
Distilled water (to make) 1,000 ml
* Make final volume up to 1.0 L with filtered tertiary distilled water.
CONWAY Trace Metal Solution
ZnCl2 2.1 g/L dH2O
CoCl2 · 6H2O 2.1 g/L dH2O
(NH4)6 Mo7O24 · 4H2O 2.1 g/L dH2O
CuSO4 · 5H2O 2.0 g/L dH2O
* Distilled water 100㎖ (acidify with 1N HCl until solution is clear)
CONWAY Vitamin Solution
Vitamin B1 20mg
Vitamin B12 10mg
Distilled water 200㎖
References
Guillard R.R.L. and Ryther J.H. (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula
confervacea Cleve. Can. J. Microbiol. 8: 229-239.
Guillard R.R.L. (1975) Culture of phytoplankton for feeding marine invertebrates.
in “Culture of Marine Invertebrate Animals.” (eds: Smith W.L. and Chanley M.H.) Plenum Press,
New York, USA. pp 26-60.
Eco-Life & Technology
Dong-Han Lee(CEO). Researcher Lab Eco life of Aquatic Envronment physiology Rsearcher of Marine Bioscience and
Technology No. 306, CATHOLIC KWANDONG UNIVERSITY BUSINESS INCUBATION CENTER R&B Park,
Saimdang-ro, Gangeung-si, Ganwon-do, Repubic of korea, South KOREA
TEL: +82-10-9112-9351 FAX: +82-33-661-4888
MAIL: ehdgo914@naver.com www.lab-ecotec.co.kr
F/2 Medium
1.0 mL NaNO3 75.0 g/L dH2O
1.0 mL NaH2PO4.H2O 5.0 g/L dH2O
1.0 mL Na2SiO3.9H2O 30.0 g/L dH2O
1.0 mL f/2 Trace Metal Solution (see recipe below)
0.5 mL f/2 Vitamin Solution (see recipe below)
Make final volume up to 1.0 L with filtered tertiary distilled water.
Note: f/2 Medium contains extensive silica precipitate and should be used only
when growing diatoms. For other algal groups use f/2-Si Medium (see below).
F/2 Vitamin Solution
(Guillard & Ryther 1962, Guillard 1975)
To 950 mL dH2O add:
Quantity Compound Stock Solution
1.0 mL Vitamin B12 (Cyanocobalamin) 1.0 g/L dH2O
10.0 mL Biotin 0.1 g/L dH2O
200.0 mg Thiamine HCl - Make final volume up to 1.0 L with dH2O.
Filter sterilize into plastic vials and store in refrigerator.
References
Guillard R.R.L. and Ryther J.H. (1962) Studies of marine planktonic diatoms.
I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol. 8: 229-239.
Guillard R.R.L. (1975) Culture of phytoplankton for feeding marine invertebrates.
in “Culture of Marine Invertebrate Animals.” (eds: Smith W.L. and Chanley M.H.) Plenum Press, New York, USA. pp 26-60.
PES = Provasoli´s enriched Seawater Medium
General purpose marine medium for xenic cultures.
Preparation: add 1 tube (20 mL) of ES-enrichment to 1000 mL of pasteurized, filtered tertiary distilled water.
For ES-enrichment solution,
add the following to 100 ml glass-distilled water:
in 100 ml glass-distilled water
NaNO3 350 mg
Na2glycerophosphate .5 H2O 50 mg
Fe-solution 25 mL
PII metal-solution 25 mL
vitamin B12 10 µg
thiamine 0.5 mg
biotin 5 µg
Tris buffer (Sigma Co.) 500 mg
* Adjust to pH 7.8, dispense in tubes (20 mL/tube) and autoclave. Store at 10° C.
Fe-solution:
Dissolve 351 mg of Fe(NH4)2(SO4)2 . 6H2O and 300 mg of Na2EDTA in 500 mL of de-ionized or distilled water
PII metal solution:
in 100 ml glass-distilled water
Na2EDTA 100 mg
H3BO3 114 mg
FeCl3 6H2O 4.9 mg
MnSO4 H2O 16.4 mg
ZnSO4.7 H2O 2.2 mg
CoSO4. 7 H2O 0.48 mg
References
Provasoli L (1968). Media and prospects for the cultivation of marine algae. In A. Watanabe, A Hattori (eds),
Cultures and Collections of Algae. Japanese Society Plant Physiology, Hakone: 63-75.
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